Normally no bacteria can infect the blood line and transfusion infected shunts , cardiac postprotezarilor bacterial endocarditis . In addition , the bacteria may invade the lymphatic vascular system through the thoracic duct , from the development of localized infections in various tissues .
Blood culture is indicated to detect invasion ( bacteremia ) and systemic spread ( septicemia ) by blood . In these cases the blood is collected by venipuncture at the bedside and then the blood is transferred into two containers : one containing anaerobic and aerobic environment containing one . Containers are kept in incubator allowing all organisms present in the blood to rise in their environment. Blood culture allows the identification of about 67 % of the pathogens in the first 24 hours and 90% within 72 hours.
Opinions on hemoculturii are divided at harvest . Some believe that during harvest is questionable and irrelevant. Others point to a collection of three samples within one hour . The first one should be obtained first symptoms by which bacterimie or septicemia is suspected . When it is suspected endocarditis due to the bacterial infection will be collected every 3 or 4 samples each from 5 to 30 minutes prior to start of antibiotic .
- forests with alcohol and iodine-based disinfectant
- syringe of 10 ml , 2-5 ml for adults and for children
- 3 or 4 needles
- containers aerobic and anaerobic culture media
- Laboratory analysis request form
- transport containers analyzes
- adhesive bandage
will check the expiration date of culture media containers .
confirm the identity of the patient ( to avoid confusion and to never take another patient analyzes ) .
be communicated to the patient what will be done , I will explain the procedure to reduce anxiety and ensure the cooperation. I will explain to the patient that will be required three blood samples at different time intervals .
Wash hands thoroughly and put gloves
install tourniquet proximal to the area chosen for the puncture
chosen area disinfected with alcohol pads to spot it stays clean . Clean the area is from the inside out to prevent contamination of existing flora puncture area surrounding skin.
not combined with the use of alcohol in the forest iodine as alcohol neutralize the effect of iodine-based disinfectants .
after disinfecting the area is expected to dry before puncture
10 ml blood is collected in a syringe adult and 2 to 6 ml for children
disinfected with iodine-based forest rubber stopper of the container with culture medium
change the needle from the syringe with blood was collected
5 ml of blood is introduced into the container with the culture medium 2 ml for adults and children
blood cultures labeled container with patient data , date and time of collection , name of the doctor who indicated , the patient’s temperature at harvest , antibioterăii specify any recent diagnostic suspicion .
to download syringe , gloves and needles in specific receptacles separate
transported immediately to the laboratory sample
puncture site is recheck to see if there was hematoma . In the case in which the hematoma is to release the energy of at least 5 minutes , after which the hot compresses .
Special considerations :
each sample will be taken at specified time intervals and from different places
will avoid being harvested by hand on catheters or venous recently caught in unless it is suspected that the product catheter sepsis .
main complication of maneuver can be hematoma