Sampling by dabbing, wiping

This type of sampling with the end applicators made ​​of cotton helps to identify agents. Harvesting procedure requires minimal contamination by normal bacterial flora present in different areas of harvest.
Once the sample is collected , the applicator is placed with the end use in a sterile tube containing transport medium .

1. Harvesting tonsillar area :

  • Materials needed:
  • gloves
  • tongue depressor
  • minilanternă / light source
  • sterile applicator ends of cotton
  • sterile tube with culture medium
  • labels
  • Laboratory analysis request form

harvesting:
 confirm the identity of the patient
 explains the procedure to reduce anxiety and ensure cooperation to
 explained to the patient that may have vomiting sensation during the buffers , but the procedure will take less than a minute
 patient sits on the bed or sitting in a chair facing the nurse
 Wash hands and put gloves
 patient is asked to hold your head back . Depress the tongue with a tongue depressor , illuminates his throat to see exactly imflamatorii areas
 If the patient has vomiting sensation and language withdraws oppressors is suggested to breathe deeply . After relaxsează , insert language oppressors but less deep the first time
 tonsil areas were buffered with applicator including flammable areas or purulent
 sure we do not touch the applicator tongue, teeth and cheeks to prevent contamination to
 applicator tube immediately placed in culture medium
 contaminated waste discard gloves and wash hands
 sample labeled with patient data , date and time of harvest, the place to harvesting
 sample sent to the lab

2 . Harvest exudate nasopharynx :
Materials needed:

  • as the harvesting tonsillar
  • Optional : nasal speculum

harvesting:
 confirm the identity of the patient
 explains the procedure to reduce anxiety and ensure cooperation to
 patient explained he was feeling sneezing or vomiting but that procedure will take less than a minute
 patient sits on the bed or sitting in a chair facing the nurse
 patient is asked to blow his nose
 patient is asked to cover one nostril and breathe only the other and vice versa. Please insert the swab in nostril breathing better
 patient is asked to hold your head back . Gently insert the applicator approximately 7.5 to 10 cm by taking Septra nasal swab end . Turn the pad and remove quickly
 alternative press oppressors language English Patient and insert another applicator to the back of soft palate turning it quickly
 applicator is inserted in the tubes with the culture medium
 discard gloves and wash hands
 labeled samples , laboratory forms completed and sent along with the samples to the laboratory

3. Harvesting the secretion from the wound :
 Materials needed:

  • sterile gloves , a sterile drape
  • forests with alcohol, betadine
  • sterile forceps
  • sterile swabs
  • 10 ml syringe
  • sterile
  • sterile culture tube with transport medium
  • labels
  • Anaerobic bacteria culture tube ( containing carbon dioxide)
  • Sterile wound compresses repansarea
  • Laboratory analysis request form

harvesting:
 wash hands , prepare a sterile field , sterile gloves are put
 dressing is removed with sterile forceps
 Clean the area around the wound with alcohol or betadine to reduce the risk of contamination of the sample with bacteria in the adjacent wound
 for aerobic culture using cotton applicator end is inserted in the wound , then gently rotate immediately transferred into aerobic culture tube
 sample is sent to the lab
 waste discard contaminated gloves
 put gloves and recover other wound dressing

4. Sampling of the ear :
Materials needed:

  • gloves
  • normal saline solution
  • gauze compresses
  • sterile swab applicator
  • sterile tube with transport medium
  • labels
  • 10 ml syringe , sterile
  • Laboratory analysis request form

harvesting:
 Wash hands and put gloves
 gently clean excess secretions from patient ear normal saline solution and gauze compresses
 applicator is inserted into the ear canal and gently rotates along the channel wall , not to injure the eardrum
 Carefully withdraw applicator to avoid touching other surfaces and placed in sterile tube with transport medium
 properly labeled sample and sent to the laboratory with laboratory forms
 middle ear harvesting is done by the physician , after grooming external ear and compresses serfiziologic by puncture and aspiration of fluid with a syringe and then
 sample sent to the lab

5. Sampling of the eye:
Materials needed:

  • sterile gloves
  • sterile normal saline
  • compresses
  • sterile applicator ends of cotton
  • sterile tube with transport medium
  • dispenser sterile ring , especially for harvesting corneal cells

harvesting:
 Wash hands and put sterile gloves
 deleted and excess secretions eyes with normal saline solution and gauze compresses , inside outwards
 withdraws lower eyelid Conjunctivitis highlight bag . Turn the applicator pad over cojunctivă bândeţe taking care not to touch other surfaces
 Keep eye applicator parallel rather than perpendicular , to prevent corneal irritation due to sudden movements
 whether to harvest corneas , it will be done by a doctor using a special device with ring
 Immediately place the ring applicator device or transport medium tube
 waste discard contaminated gloves
 labeled sample and sent to the laboratory along with forms

Special considerations :
 do not use an antiseptic before harvesting to prevent eye irritation and change the likely outcome
 if the patient is a child or an adult uncooperative will be asked by a colleague to keep your head still during harvest , thus preventing eye damage due to sudden movements

6. Sampling of anus
Materials needed:

  • gloves
  • soap and water
  • sterile applicator end of the towel cotton
  • normal saline solution
  • sterile culture tube with transport medium
  • labels
  • Laboratory analysis request form

harvesting:
 Wash hands and put gloves
 explains the procedure to reduce anxiety and ensure cooperation to
 ensure patient privacy
 perianal wash patient with soap and water
 applicator is inserted in the end of cotton ( pre- soaked in normal saline solution ) into the anus 1 cm to 4 cm for children and adults and gently rotated around it
 applicator is inserted into the tube culture transport medium
 contaminated waste discard gloves and wash hands
 labeled sample and sent to the laboratory with the form

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